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Transcription regulation by transcription factors. Fluorescent signal of the SPRINT reactions was measured at 20 minutes reaction time. Background was subtracted and signal normalized to 125 nM fluorescein. Bars indicate mean value, error bars indicate s.d. from the mean. n = 3. (A) SmtB de-represses transcription in response to zinc, but not copper. (B) TetR de-represses transcription in response to anhydrotetracycline. *(54).
