Figure 3.

Deletion of transmembrane domain (TMDs) within the prM protein abrogates the effect of RPLP1/2 knockdown on viral protein expression. (A) Tetracycline inducible HeLa cells expressing DENV structural proteins were transfected with control or RPLP1/2 siRNAs and levels of HA-C-prM and EΔ208-FLAG were analyzed by western blot using the respective tag antibodies. Analysis of cells expressing variants lacking TMD3 (B) or both TMD 2 and 3 (C) is shown. Representative experiments and shown and their quantifications are shown on the right. The graphs show mean values ± SD. Asterisks indicate p values: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.