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. 2020 Sep 5;48(17):9872–9885. doi: 10.1093/nar/gkaa717

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — RPLP1/2 knockdown increases the rates of DENV structural protein synthesis and turnover. HeLa cells expressing HA-C-prM-EΔ208-FLAG were transfected with control or RPLP1/2 siRNAs and then metabolically pulse-labeled with ³⁵S-methionine/cysteine for 30 min followed by chase with cold methionine/cysteine. Cells were harvested immediately after the pulse, 3 or 6 hours after the chase for lysis and IP with a-HA and a-FLAG antibodies. (A) An autoradiogram of triplicate IP samples for HA-C-prM and EΔ208-FLAG is shown for the pulse (0 h) and chase samples. Controls in the two leftmost lanes were not induced with tetracycline. (B) The left panel shows raw quantification of band intensities and the right panel shows levels normalized to the 0 h time point for HA-C-prM. (C) Same as in (B) except data show EΔ208-FLG. A representative experimenti is shown and its quantifications were done by averaging the measurements of triplicate bands in the autoradiagram.