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. 2020 Aug 7;48(17):9538–9549. doi: 10.1093/nar/gkaa663

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — FRET-based approach for studying the effects of histone H4K77succ on nucleosome stability. Plot shows the normalized FRET intensity versus salt concentration in unmodified (black trace) and H4K77succ (red trace) nucleosomes labeled by FRET pairs at DNA ends (A) and H2B-DNA⁻¹⁵ (B). The salt concentration at which the FRET decreases by 50% is denoted as C_1/2. For visualization, all curves were normalized between 100% at the maximum FRET value and 0 at high salt concentration. Left panel, a representative salt titration plot of the unmodified and H4K77succ nucleosomes. Salt titrations were repeated three times on nucleosomes from one preparation (n = 3, C_1/2 = mean ± s.e.m). Middle panel, the cartoon indicates the relative locations of labels on the nucleosome and the process of salt-induced nucleosome dissociation. The C_1/2 values of independent experiments in each type of nucleosome are showed. The average of C_1/2 (mean ± s.e.m.) was calculated from three biological replicates (independent nucleosome preparations). The decrease in C_1/2 value was calculated as . Right panel, histogram shows the change in nucleosome stability associated with succinylation modification. The mean of the C_1/2 values of unmodified nucleosomes was set to 100%. The P values were based on a two-tailed Student's t test. **P < 0.01.

Inline graphic — FRET-based approach for studying the effects of histone H4K77succ on nucleosome stability. Plot shows the normalized FRET intensity versus salt concentration in unmodified (black trace) and H4K77succ (red trace) nucleosomes labeled by FRET pairs at DNA ends (A) and H2B-DNA⁻¹⁵ (B). The salt concentration at which the FRET decreases by 50% is denoted as C_1/2. For visualization, all curves were normalized between 100% at the maximum FRET value and 0 at high salt concentration. Left panel, a representative salt titration plot of the unmodified and H4K77succ nucleosomes. Salt titrations were repeated three times on nucleosomes from one preparation (n = 3, C_1/2 = mean ± s.e.m). Middle panel, the cartoon indicates the relative locations of labels on the nucleosome and the process of salt-induced nucleosome dissociation. The C_1/2 values of independent experiments in each type of nucleosome are showed. The average of C_1/2 (mean ± s.e.m.) was calculated from three biological replicates (independent nucleosome preparations). The decrease in C_1/2 value was calculated as . Right panel, histogram shows the change in nucleosome stability associated with succinylation modification. The mean of the C_1/2 values of unmodified nucleosomes was set to 100%. The P values were based on a two-tailed Student's t test. **P < 0.01.