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. 2020 Jul 7;48(17):9415–9432. doi: 10.1093/nar/gkaa544

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — ELYS binds the acidic patch using redundant basic sequences. (A) Y-complex composition with proteins identified to be acidic patch-dependent by mass spectrometry and/or western blot indicated. (B) Schematic of ELYS indicating functional regions and ELYS^C sequence with Arg and Lys mutated to Ala in ELYS^Cx1-x6. (C) Pulldown of ELYS^C by WT or acidic patch mutant (AP mut) FLAG-tagged nucleosomes alone or with WT or nucleosome-binding deficient mutant of LANA (LANA mut) added as a competitor. (D) Pulldown of ELYS^C or ELYS^C mutants by WT and AP mut nucleosomes. Flowthrough in panels C and D demonstrate equivalent loading of ELYS or LANA variants, respectively.