Figure 6.

ELYS requires redundant basic sequences for chromatin recruitment. (A and B) Interphase and metaphase images of HeLa cells expressing exogenous WT or mutant GFP-ELYS proteins. (C) Frequency of localization phenotypes, >100 cells scored for each GFP-ELYS variant. Statistical significance determined using Fisher's Exact Test with Bonferroni correction for multiple testing. (NS = not significant; **** indicates P < 0.0001). (D) Localization profiles of GFP-ELYS mutants across the metaphase plate for cells in panel (C). Line and shaded areas represent mean ± 1 standard deviation. (E) Confocal images of metaphase cells.