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. 2020 Aug 20;39(39):6190–6202. doi: 10.1038/s41388-020-01422-9

Fig. 4. Overexpression of miR-450a-5p strengthens autophagy in glioma cells.

Fig. 4

a The expressions of autophagy-related proteins determined by Western blot analysis. b Quantitative analysis of the Western blot results. c The autophagosomes in both cell lines visualized by labeling the LC3. d The colocalization of LC3 and LAMP2 by immunofluorescence study. In all assays, the transfected cells were cultured with DMEM medium containing gefitinib (20 μM for A172 cells and 40 μM for SHG-44 cells) for 48 h. The result was a representative of three independent experiments. Note: Error bars represented mean ± SD. p values were determined by one-way analysis of variance (ANOVA) followed by Tukey post hoc test. ***p < 0.001, **p < 0.01 and *p < 0.05.