Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 20;39(39):6190–6202. doi: 10.1038/s41388-020-01422-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Effect of WIPI1 knockdown on the glioma cell proliferation tested by the colony formation assay. b Effect of WIPI1 knockdown on the glioma cell apoptosis detected by the flow cytometry. c Effect of WIPI1 knockdown on the invasion abilities of glioma cells detected by the Transwell invasion assay. d Effect of WIPI1 knockdown on the migration abilities of glioma cells detected by the wound healing assay. e Quantitative analysis of the numbers of colonies. f Quantitative analysis of apoptosis rate. g Quantitative analysis of the number of invaded cells. h Quantitative analysis of the migration rate. The transfected cells were treated with gefitinib (20 μM for A172 cells and 40 μM for SHG-44 cells). In the colony formation assay, the cells were cultured for 2 more weeks. In all other assays, the transfected cells were cultured for 48 h. The result was a representative of three independent experiments. Note: Error bars represented mean ± SD. p values were determined by one-way analysis of variance (ANOVA) followed by Tukey post hoc test. ***p < 0.001, **p < 0.01 and *p < 0.05.