Fig. 2. FcγRIIb knockout reduces leukemic clonogenic potential and cell proliferation.

a Bcr-Abl and ev (empty vector) infected lin⁻ BM cells from FcγRIIb^+/+ and FcγRIIb^−/− (1000 cells/ml) were seeded in methylcellulose with or without cytokines. Numbers of colonies (colony-forming units, CFUs) were assessed after 7 days. Re-plating was performed using 1 × 10⁴ cells. CFU numbers were counted after 7 days. b Proliferation assay using FcγRIIb^+/+ and FcγRIIb^−/− Bcr-Abl⁺ cells was performed using Trypan blue exclusion method. c Cell cycle was assessed in FcγRIIb^+/+ and FcγRIIb^−/− Bcr-Abl⁺ cells. Data are shown as mean ± SD. n = 3, each, *p < 0.05, **p < 0.001, ***p < 0.0001, and n.s. not statistically significant.