Table 2.

Candidate reference genes for qPCR.

Gene (NCBI accession number)	Primer sequence	Amplicon length (bp)	Ta (°C)	Tm (°C)
60S (XM_002270599.3)	Fw: ATCTACCTCAAGCTCCTAGTC Rev: CAATCTTGTCCTCCTTTCCT	165	60	79.6
TTC7B (XM_002283371.4)	Fw: GCTCTGTTGTTGAAGATGGG Rev: GGAAGCAGTTTGTAGCATCAG	156	60	79.9
EF1α (XM_002284888.3)	Fw: GAACTGGGTGCTTGATAGGC Rev: ACCAAAATATCCGGAGTAAAAGA	164	60	79.7
UBQ (XR_002030722.1)	Fw: GCCCTGCACTTACCATCTTTAAG Rev: GAGGGTCGTCAGGATTTGGA	75	60	78.9
SAND (XM_002285134.3)	Fw: CAACATCCTTTACCCATTGACAGA Rev: GCATTTGATCCACTTGCAGATAAG	76	60	79.2
GAPDH (XM_002263109.3)	Fw: TCAAGGTCAAGGACTCTAACACC Rev: CCAACAACGAACATAGGAGCA	226	60	81.3
ACTINa (XM_019223591.1)	Fw: ATTCCTCACCATCATCAGCA Rev: GACCCCCTCCTACTAAAACT	89	55	77.5
α-TUB (XM_002285685.4)	Fw: CAGCCAGATCTTCACGAGCTT Rev: GTTCTCGCGCATTGACCATA	119	60	78.8
AP2M (XM_002281261.3)	Fw: CCTCTCTGGAATGCCTGATTT Rev: CTTTAGCAGGACGGGATTTA	89	55	75.0
β-TUB (XM_002275270.3)	Fw: TGAACCACTTGATCTCTGCGACTA Rev: CAGCTTGCGGAGGTCTGAGT	86	60	82.3

Genes, gene accession numbers, primer sequences (Fw, forward; Rev, reverse), amplicon length (bp, base pairs) and qPCR annealing (Ta) and melting (Tm) temperatures are indicated.

^aAlternative splicing variant.