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. 2020 Sep 24;9(9):84. doi: 10.1038/s41389-020-00268-w

Fig. 2. Assay for the interaction between β2-AR and YB-1.

Fig. 2

a Immunoprecipitation for β2-AR using anti-β2-AR and anti-YB-1 antibodies, respectively, in HCC specimens with metastasis (left, upper). Immunoprecipitation for YB-1 using anti-β2-AR and anti-YB-1 antibodies, respectively, in HCC specimens with metastasis (left, lower). Immunoprecipitation for β2-AR using anti-β2-AR and anti-YB-1 antibodies, respectively, in HCC specimens without metastasis (right, upper). Immunoprecipitation for YB-1 using anti-β2-AR and anti-YB-1 antibodies, respectively, in HCC specimens without metastasis (right, lower). b Immunoprecipitation for β2-AR using anti-β2-AR and anti-YB-1 antibodies, respectively, in SMMC-7721 cells pre-treated with 10 μM ISO (left, upper). Immunoprecipitation for YB-1 using anti-β2-AR and anti-YB-1 antibodies, respectively, in SMMC-7721 cells pre-treated with 10 μM ISO (Left, Lower). Immunoprecipitation for β2-AR using anti-β2-AR and anti-YB-1 antibodies, respectively, in SMMC-7721 cells without ISO treatment (right, upper). c Glutathione S-transferase (GST) pulldown of HA-β2-AR from whole-cell extracts of HEK293T cells transfected with GST or GST-YB-1 fusion proteins. d Association between ectopically expressed β2-AR and YB-1 in HEK293T cells. e Schematic diagram of full-length YB-1 and the generated deletion mutants. Co-IP for HA-β2-AR with YB-1 truncation mutants (upper). Western blotting of HA-β2-AR and YB-1 truncation mutants (middle and lower). f Schematic diagram of full-length β2-AR and the generated deletion mutants. Co-IP for Myc-YB-1 with β2-AR mutants (upper). Western blotting of Myc-YB-1 and β2-AR truncation mutants (middle and lower).