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. 2020 Sep 24;11:4820. doi: 10.1038/s41467-020-18629-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–b Active site close-ups of the evolved sTyrRS (a, PBD ID: 6WN2) and EcTyrRS (b, PDB ID: 1X8X). The active site surfaces are in cyan. Four residues included in library construction are labeled. c–d Recognition of ligands by sTyrRS (c) and the wild-type EcTyrRS (d). Carbon atoms in ligands are shown in green. Carbon atoms in proteins are shown in silver. All oxygen atoms are in red, nitrogen atoms are in blue.