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. 2020 Sep 24;11:4820. doi: 10.1038/s41467-020-18629-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Western blot analysis of CXCR4(N38-21TAG)-EGFP expression in the absence (lane 1) and presence of sTyr (1 mM, lane 2) in 293T cells with psTyrRS. b Western blot analysis of partially purified CXCR4(N38)-EGFP variants. Lane 1, CXCR4(N38)-EGFP; lane 2, CXCR4(N38-FFF)-EGFP; lane 3, CXCR4(N38-FF 21TAG)-EGFP in the absence of sTyr; lane 4, CXCR4(N38-FF 21TAG)-EGFP in the presence of sTyr (1 mM); c Calcium mobilization assay. wt, wild-type CXCR4; FFF, CXCR4(FFF) mutant; FF TAG, CXCR4(FF 21TAG) mutant. Lane M in both (a) and (b) is molecular weight marker. c Data are plotted as the mean ± s.d. from n = 3 independent experiments. a–c Source data are provided as a Source data file.