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. 2020 Sep 24;11:4837. doi: 10.1038/s41467-020-18607-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Bone marrow-derived macrophages (BMDM) were derived from exon 2 floxed (Mfsd7c^fl/fl) C57BL/6 mice and C57BL/6 mice with exon 2 deletion in macrophages (Mfsd7c^−/−). a, b Representative OCR analysis from Mfsd7c^fl/fl and Mfsd7c^−/− macrophages as measured by Seahorse XF96e Analyzer and their responses to oligomycin (oligo), FCCP, and rotenone plus antimycin A (Rot/AA) (n = 3 technical replicates) (a), and their average basal and maximal OCR (b) (n = 9 independent experiments from 3 biological replicates). c Representative ECAR analysis of Mfsd7c^fl/fl and Mfsd7c^−/− macrophages and their responses to glucose, Rot/AA, and 2-deoxyglucose (2-DG) (n = 3 technical replicates). d Mitochondrial membrane potential of Mfsd7c^fl/fl and Mfsd7c^−/− macrophages analyzed with TMRE staining followed by flow cytometry (1 representative histogram picked from 3 biological replicates). e Cellular ATP/ADP ratio, (n = 4 biological replicates from average of 5 technical replicates). f Estimates of cellular temperature of Mfsd7c^fl/fl and Mfsd7c^−/− macrophages as measured by fluorescent thermoprobe (n = 3 biological replicates). p values were calculated using unpaired t-test (*p < 0.05, **p < 0.01, ***p < 0.005). Data are presented as mean value ± standard deviation.