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. 2020 Sep 11;11:580968. doi: 10.3389/fimmu.2020.580968

FIGURE 1.

FIGURE 1

Key NAFLD associated pathologies were recapitulated in HIL mice fed with HFHC diet. Ten to 12-week-old HIL mice were given ad libitum access to HFHC diet over a period of 20 weeks. (A) Weight gain of HIL mice fed with chow diet and HFHC diet over 20 weeks. Weight gain was determined after normalizing to weight at week 0. Data are presented as mean weight gain ± SEM. Two-tailed Mann–Whitney U test; **p < 0.01, ***p < 0.001. (B) Accumulation of abdominal fat and evidence of severe liver damage in HIL mice fed with HFHC diet. Images are representative of each diet group at week 20. Scale bar: 1 cm. (C) Comparison at week 20 of NAFLD score and % area of liver fibrosis in HIL mice fed with chow (n = 9) or HFHC diet (n = 21). Data are presented as mean ± SEM. Two-tailed Mann–Whitney U test; ***p < 0.001. (D) H&E, Fast Green/Sirius Red (FG/SR) and Oil Red O staining of liver sections from HFHC-fed HIL mice at weeks 0, 4, 8, 12, 16, and 20 of diet treatment. Images are representative of 3–6 HIL mice per time point. Scale bar: 100 μm. cv, central vein; pv, portal vein. (E) TUNEL staining of liver sections from chow-fed and HFHC-fed HIL mice at 20 weeks. Scale bar: 100 μm. (F) Positive correlation between presence of activated hepatic stellate cells (marked by α-SMA) and proportion of fibrotic region (FG/SR staining) in liver of HIL mice at 20 weeks. Scale bar: 100 μm.