FIGURE 1.

Cell numbers of uDCs after coitus in allogeneic mating. (A) Experimental time course. The DCs in the uterus in non-mated control virgin mice and mice on days 0.5, 1.5, 2.5, and 3.5 pc were analyzed by flow cytometry. (B) Gating strategy was used to identify CD103^– DCs, CD103⁺ DCs, and pDCs in the uterus. (C) Graphs show number of CD11c⁺ DCs and each DC subset in the uterus at each time point. A minimum of five samples from each time point were analyzed. Data represent mean ± SEM and are representative of three independent experiments. Statistical comparisons were performed using Kruskal-Wallis test with Dunn’s multiple comparisons test (^∗∗P < 0.01, ^∗P < 0.05).