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. 2020 Sep 11;11:557720. doi: 10.3389/fimmu.2020.557720

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Copyright © 2020 Yasuda, Shima, Moriya, Ikebuchi, Kusumoto, Ushijima, Nakashima, Tomura and Saito.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of uDC phenotype between allogeneic- and syngeneic mating. (A) Flow cytometry contour plots show immature DCs and mature DCs in syngeneic mating on days 1.5 and 3.5 pc. (B,C) Graphs show total DC number (B), proportion of immature DCs (C) and mature DCs (D) out of the total DCs, and the ratio of mature DCs/immature DCs within each DC subset (E) in virgin, allogeneic-, and syngeneic mating mice at each time point. A minimum of five samples from each time point were analyzed. Data represent mean ± SEM (B,C) and are representative of three independent experiments. Statistical comparisons were performed using Mann-Whitney U-test (^∗∗P < 0.01, ^∗P < 0.05).