FIGURE 4.

Time course for migratory DCs and remaining DCs in the uterus and dLNs after coitus in allogeneic mating. (A) Experimental time course. Virgin mice were synchronized at the diestrous stage, and the uteri of mice after coitus in allogeneic mating were photoconverted on days 0.5, 1.5, and 2.5 pc. The DCs in the uteri and dLNs were analyzed 24 h after photoconversion. (B–E) Graphs show the ratio of mature DCs/immature DCs (B,C) in each DC subset labeled with KikGR-green (B) and KikGR-red (C), and proportion of each DC subset labeled with KikGR-green in the uterus, (D) and KikGR-red within MHC class II high DC subset in the dLNs (E) out of the total DCs 24 h after photoconversion. Three samples from each time point were analyzed. Data represent mean ± SEM (B–E) and are representative of three independent experiments. Statistical comparisons were performed using the Kruskal-Wallis test with Dunn’s multiple comparisons test.