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. 2020 Sep 11;11:557720. doi: 10.3389/fimmu.2020.557720

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Copyright © 2020 Yasuda, Shima, Moriya, Ikebuchi, Kusumoto, Ushijima, Nakashima, Tomura and Saito.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of PD-L2 in uDCs increased immediately before implantation. (A) Flow cytometry contour plots show expressions of PD-L2 in uterine CD11c⁺ DCs with fluorescence minus one (FMO). (B,C) Proportion of PD-L2⁺ DCs of total DCs (B), immature DCs, and mature DCs (C) in allogeneic mating at each time point. (D,E) Proportion of PD-L2⁺ DCs within the uterus (D) and dLNs (E) in allogeneic mating 24 h after uterine photoconversion on day 3.5 pc. A minimum of five (B,C) and four (D,E) samples from each time point were analyzed. Data represent mean ± SEM (B,C) and are representative of three independent experiments. Statistical comparisons were performed using Mann-Whitney U-test (B,C) and Kruskal-Wallis test with Dunn’s multiple comparisons test (D,E) (^∗∗P < 0.01, ^∗P < 0.05).