FIGURE 6.

The importance of MepM in adaptation to salt stress. (A) The effect of simultaneous overexpression of MepS, MepH, and PbpG on salt sensitivity of the mepM mutant. The cells of the wild-type, mepM mutant, and mepM mutant harboring the indicated plasmids were serially diluted from 10⁸ to 10⁴ cells/mL in 10-fold steps and spotted onto an LB plate or LB plates containing 750 mM NaCl and the indicated concentrations of arabinose. (B) The effect of the mepS mepH double or mepS mepH pbpG triple deletion on adaptation to salt stress. The wild-type and the indicated mutant cells were serially diluted from 10⁸ to 10⁴ cells/mL in 10-fold steps and spotted onto an LB plate or LB plates containing 750 or 800 mM NaCl as indicated.