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. 2020 Sep 11;11:572960. doi: 10.3389/fimmu.2020.572960

FIGURE 2.

FIGURE 2

The RLR-mediated shift to glycolysis is impaired upon mTOR inhibition in moDCs. Immature moDCs were pre-treated with vehicle control, 100 nM rapamycin (RAPA) or 100 nM AZD8055 (AZD) for 2 h and then stimulated with 3p-hpRNA (0.5 μg/ml) (A,C) or polyI:C (1 μg/ml) (B,D) for 12 h. (A,B) The expression of LDHA, HK2 and HIF1A was assessed at the mRNA level by real-time PCR. Bar graphs represent the mean ± SD of 4 independent experiments. (C,D) Lactate concentrations were measured from cell culture supernatants. Bar graphs represent the mean ± SD of 6 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.01, ****p < 0.0001 vs. control; ##p < 0.01, ###p < 0.001, ####p < 0.0001.