FIGURE 3.

The RLR-mediated production of IFN-α and pro-inflammatory cytokines is decreased upon mTOR blockade in moDCs. Immature moDCs were pre-treated with vehicle control, 100 nM rapamycin (RAPA) or 100 nM AZD8055 (AZD) for 2 h and then stimulated with 3p-hpRNA (0.5 μg/ml) (A), polyI:C (1 μg/ml) (B) or VSV (MOI 1) (C). (A–C) IFN-α, IL-6 and TNF protein levels were measured by ELISA 24 h after 3p-hpRNA and polyI:C activation as well as 18 h after VSV stimulation. Data are shown as mean ± SD from 5 to 8 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.01, ****p < 0.0001 vs. control; ^###p < 0.001, ^####p < 0.0001. ND, not determined.