FIGURE 4.

RLR stimulation enhances TBK1 activity in moDCs that is decreased by rapamycin and AZD8055 pre-treatment. Immature moDCs were pre-treated with vehicle control, 100 nM rapamycin (RAPA) or 100 nM AZD8055 (AZD) for 2 h then stimulated with 3p-hpRNA (0.5 μg/ml) (A,C) or polyI:C (1 μg/ml) (B,D) for different time periods. Kinetics of TBK1 phosphorylation were determined by western blotting. (A,B) Representative blots are shown. (C,D) Bar graphs represent the mean ± SD of at least 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.01, ****p < 0.0001 vs. control; ^##p < 0.01, ^####p < 0.0001.