FIGURE 5.

RLR stimulation increases mTORC1 and mTORC2 activity in GEN2.2 cells that can be effectively inhibited by rapamycin and AZD8055 pre-conditioning. GEN2.2 cells were pre-treated with vehicle control, 100 nM rapamycin (RAPA) or 100 nM AZD8055 (AZD) for 2 h and then stimulated with 3p-hpRNA (0.5 μg/ml) (A,B) or polyI:C (1 μg/ml) (C,D) in a time-dependent manner. Kinetics of p70S6K and Akt phosphorylation was determined by western blotting. (A,C) Representative blots are shown. (B,D) Bar graphs represent the mean ± SD from 4 independent experiments. Specific bands for Akt are indicated by arrows. *p < 0.05, **p < 0.01, ***p < 0.01, ****p < 0.0001 vs. control; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001. ND, not determined.