FIGURE 7.

RLR stimulation increases the phosphorylation of TBK1 that is reduced by rapamycin and AZD8055 pre-treatment in GEN2.2 cells. Cells were pre-treated with vehicle control, 100 nM rapamycin (RAPA) or 100 nM AZD8055 (AZD) for 2 h then stimulated with 3p-hpRNA (0.5 μg/ml) (A,C) or polyI:C (1 μg/ml) (B,D) for different time periods. Kinetics of TBK1 phosphorylation were determined by western blotting. (A,B) Representative blots are shown. (C,D) Bar graphs represent the mean ± SD of at least 3 independent experiments. **p < 0.01, ****p < 0.0001 vs. control; ^#p < 0.05, ^###p < 0.001, ^####p < 0.0001. ND, not determined.