FIGURE 8.

The RLR-stimulated secretion of IFN-α and pro-inflammatory cytokines is abolished upon mTOR blockade in primary human pDCs. Primary pDCs were pre-treated with vehicle control, 100 nM rapamycin (RAPA) or 100 nM AZD8055 (AZD) for 2 h and then stimulated with 3p-hpRNA (0.5 μg/ml) (A) or polyI:C (1 μg/ml) (B). (A,B) IFN-α, IL-6 and TNF protein levels were measured by ELISA 6 h after stimulation. Data are shown as mean ± SD from 3 independent experiments. **p < 0.01, ***p < 0.01, ****p < 0.0001 vs. control; ^#p < 0.05, ^##p < 0.01, ^####p < 0.0001. ND, not determined.