FIGURE 9.

Rapamycin but not AZD8055 effectively inhibits the ability of RLR-stimulated moDCs and primary pDCs to induce proliferation of naive T cells. CFSE-labeled naïve CD8 + T cells were co-cultured with allogeneic moDCs (A–D) and primary pDCs (E–H) pre-treated with the indicated reagents. After 5 days of co-cultivation, cell division was measured by flow cytometry. (A,C,E,G) Representative histograms are shown where numbers indicate the percentage of viable dividing T cells. In A and C T cell controls are the same, since representative dot plots originate from the same set of experiment from one single donor. (B,D,F,H) Bar graphs represent the mean ± SD of at least 6 independent experiments. *p < 0.05, **p < 0.01, ****p < 0.0001 vs. control; ^###p < 0.001, ^####p < 0.0001. AZD: AZD8055, RAPA: rapamycin.