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Immunofluorescent Analysis of the Expression of β-Dystroglycan
Immunofluorescent analysis of the expression of sarcolemmal β-dystroglycan on diaphragm cryosections (scale bar, 50 μm). β-Dystroglycan was visualized by anti-β-dystroglycan (Santa Cruz) labeled with Zenon Alexa Fluor 488 dye (Thermo Fisher Scientific). Images were captured under the Nikon microscope with the NIS-Elements software (Nikon Instruments). C57, C57BL/10ScSn; mdx, C57BL/10ScSnmdx.
