Figure 1.
circRNA Profiling in Human HCC Tissues and circTMEM45A Characterization
(A) Among the 981 differentially expressed circRNAs, 289 circRNAs were verified as novel circRNAs, and 692 circRNAs were identified beforehand and listed in the circRNA database. (B) The number of upregulated (red) and downregulated (green) circRNAs according to their categories of formation mode. (C) The heatmap shows the top 10 dysregulated circRNAs between HCC tissues and adjacent normal samples. (D) Expression levels of top 10 dysregulated circRNAs were measured by quantitative real-time PCR. (E) Comparison of log2 fold changes (FCs) in circRNAs between circRNA microarrays and quantitative real-time PCR results. (F) The level of circTMEM45A was significantly increased in HCC samples compared to adjacent normal samples. (G) Evaluation of the diagnostic performance of circTMEM45A for HCC diagnosis. (H) Kaplan-Meier curve revealed that high expression of circTMEM45A was relative to a poor overall survival in HCC patients. (I) The levels of circTMEM45A were significantly increased in HCC cell lines compared to the normal liver cell line L02. All tests were performed at least three times. Data are expressed as mean ± SD. ∗∗p < 0.01, ∗∗∗p < 0.001.