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. 2020 Sep 11;11:1015. doi: 10.3389/fgene.2020.01015

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Copyright © 2020 Sanei-Ataabadi, Mowla and Nasr-Esfahani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of relative expression levels of various SLC7A11-AS1 isoforms in spermatozoa of individuals with varicocele with those of fertile men. (A) Schematic representation from eight isoforms of SLC7A11-AS1. Five primer pairs (I–V) were used for evaluation of expression in spermatozoa samples (primers were indicated by arrows; qPCR-amplified products were indicated by red boxes). (B) Quantitative RT-PCR analysis of the expression of SLC7A11-AS1 isoforms using five primer pairs in the ejaculated spermatozoa of varicocele and control groups. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used for expression normalization. *P < 0.05 and ***p < 0.001 using independent sample t-test.