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. 2020 Sep 11;11:1015. doi: 10.3389/fgene.2020.01015

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Copyright © 2020 Sanei-Ataabadi, Mowla and Nasr-Esfahani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment of oxidative stress markers after overexpression of SLC-AS6. (A) Transfecting SLC-AS6 diminished the glutathione (GSH) levels significantly relative to mock-transfected cells in NT2 and NCCIT cell lines. (B) Cytosolic reactive oxygen species (ROS) levels were elevated significantly after overexpression of SLC-AS6 in NT2 and NCCIT cells. (C) Transfecting SLC-AS6 in NT2 cell line leads to an increase in lipid peroxidation detected by C11-BODIPY staining. Error bars show standard error of mean (SEM) of triplicated experiments (*P < 0.05 and **P < 0.01 using independent sample t-test).