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. 2020 Sep 11;11:1015. doi: 10.3389/fgene.2020.01015

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Copyright © 2020 Sanei-Ataabadi, Mowla and Nasr-Esfahani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of SLC-AS6 overexpression on cell viability. (A) Percentages of apoptotic (Annexin-positive, PI-negative) and necrotic cells (Annexin- and PI-positive) were elevated significantly in NT2 and NCCIT cell lines transfected with SLC-AS6. (B) MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)- 2-(4-sulfophenyl)-2H-tetrazolium] assay results showed that overexpression of SLC-AS6 significantly decreased survival rate of NT2 and NCCIT cells lines. Error bars show standard error of mean (SEM) of triplicated experiments (*P < 0.05 using independent sample t-test).