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. 2020 Sep 11;11:868. doi: 10.3389/fphys.2020.00868

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Copyright © 2020 Wen, Liu, Li, Lin, Chen, Zhou, Tang, Wang and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ER stress and autophagy regulate goat luteal cell apoptosis induced by PGF2α. (A–F) Cells cultured in medium with PBS, PGF2α (1 μM), PGF2α (1 μM), and 4-PBA (1 μM) to inhibit ERS or PGF2α (1 μM) and CQ (100 μM) to inhibit autophagy,4-PBA (1 μM) and CQ (100 μM) for 24 h are stained with annexin V-FITC/PI and analyzed by flow cytometry. (G) The histogram shows the apoptotic rate displayed in A-F from three separate experiments (N = 3). Means are compared with one-way ANOVA combined with Tukey’s post hoc tests. ^∗∗p< 0.01, ^∗∗∗p< 0.001.