FIGURE 5.

Effect of EIF2S1 knockdown on goat luteal cell apoptosis, ER stress, and autophagy under PGF2α treatment. (A) Cells cultured in medium with PGF2α (1 μM) (b,d) or isopycnic PBS (a,c) for 24 h are stained with annexin V-APC/PI and analyzed by flow cytometry. (e) The histogram shows the apoptotic rate displayed in (a–d) from three separate experiments. (B) The changes in the expression of p-EIF2S1, ATF6, LC3, and p-IRE1 under EIF2S1 knockdown. The goat luteal cells infected with a lentivirus specific for EIF2S1 and a negative lentivirus (shNC) for 48 h. Then, cells are treated with PGF2α (1 μM) for 24 h, total protein from LCs were subjected to western blot analysis. Data in the bar graph represent the mean ± SEM of three independent experiments (N = 3). Means are compared with one-way ANOVA combined with Tukey’s post hoc tests. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.