Table 1.
The nucleotide sequences of the primer pairs used in this study.
| Primer | Sequence (5’- 3’) |
|---|---|
| ZmMYB59-F | ATTGAGCTCCATGCTCGGTG |
| ZmMYB59-R | TAGCTGAGTGGCCTGACCAA |
| ZmMYB59RT-F | CTGTCCGCCTGTTTGGTG |
| ZmMYB59RT-R | CAGCCTCCTTGCTATCCTAG |
| OsActin-F | AGTGTCTGGATTGGAGGAT |
| OsActin-R | TCTTGGCTTAGCATTCTTG |
| NtActin7-F | ACTTTCCAGTGACCTCTTTCCG |
| NtActin7-R | CAGCAAATCCAGCCTTCACCA |
Primer ZmMYB59F/ZmMYB59R was used for genomic identification. Primer ZmMYB59RT-F /ZmMYB59RT-R was used for detecting expression of ZmMYB59 expression level. Primers OsActin-F /OsActin-R, NtActin7-F /NtActin7-R were used for internal references during semi-quantitative RT-PCR and qRT-PCR.