FIGURE 4.

Impact of washing on lipid extraction (A) and Yo-Pro uptake (B). Two different washing techniques were implemented. In technique A, distilled water was added to reduce conductivity in yeast broth prior to centrifugation and in technique B, the yeast suspension was centrifuged beforehand, subsequently the biomass was resuspended to the same concentration as before with distilled water supplemented with NaCl to reach the targeted conductivity. Results are average ± standard deviation of two independent experiments (n = 2) with duplicates in each. Control refer to yeast processed directly after harvest i.e., not submitted to any washing treatment. Statistical significances are indicated in comparison with control.