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. 2020 Sep 11;8:575379. doi: 10.3389/fbioe.2020.575379

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Copyright © 2020 Gorte, Nazarova, Papachristou, Wüstner, Leber, Syldatk, Ochsenreither, Frey and Silve.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipid extraction yields obtained after processing along the “washed-route.” PEF-treatment was applied on the yeast washed after harvesting. Impact of the specific treatment energy at a constant electric field value of (A) 14 kV/cm and (B) 40 kV/cm. For the bottom graph, the gray bars are the results of lipid extraction performed directly after the PEF-treatment and the green bars are the lipid extraction yields obtained when a washing step is added after PEF-treatment before addition of extraction solvents. The yellow lines illustrate the average ± standard deviation of Soxhlet extractions, as absolute control. For both experiments, the results are reported as the average + standard deviation of three independent experiments (n = 3) with samples processed in duplicates. Statistical significances are indicated in comparison with the unwashed control.