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. 2020 Sep 11;8:1009. doi: 10.3389/fbioe.2020.01009

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Copyright © 2020 McLaughlin, Beal, Mısırlı, Grünberg, Bartley, Scott-Brown, Vaidyanathan, Fontanarrosa, Oberortner, Wipat, Gorochowski and Myers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gardner et al. (2000) toggle switch represented using SBOL versions 2 and 3. In the SBOL2 representation, the structure of the lacI and tetR units are defined in ComponentDefinitions and instantiated in a ModuleDefinition as FunctionalComponents. Their function-linked sub-structures are then mapped into the ModuleDefinition using MapsTo to assign corresponding FunctionalComponents for the promoter and CDS components, which can then be used as participants in Interactions. In the SBOL3 representation, the creation of a ModuleDefinition and MapsTo relations as in the SBOL2 example is no longer necessary as sequence information and interactions can co-exist in the same parent Component object. Diagrams are drawn using SBOL Visual notation (Beal et al., 2019). A serialized SBOL3 representation of this construct is available on GitHub at https://github.com/SynBioDex/SBOLTestSuite/tree/master/SBOL3/toggle_switch.