Figure 1.

DiY Formation in Aβ and vAβ via Metal-Catalyzed Oxidation

(A–D) Freshly prepared Aβ and vAβ (50 μM) were incubated at 37°C, without CuCl₂, with CuCl₂ (400 μM), or CuCl₂ in combination with H₂O₂. Fluorescence spectra were collected 5 min post-incubation using fluorescent excitation wavelength of 320 nm and emission collected between 340 and 600 nm, with DiY peak signal observed between 400 and 420 nm (A). Fluorescence spectra were also collected at 5 min using an excitation wavelength of 280 nm and emission collected between 290 and 500, with peak tyrosine signal observed at 305 nm (B). Fluorescence spectra were collected again at 2 h (C) and then 5 days (D) to follow DiY formation over time. A minimum of three independent experiments was repeated to ensure the reproducibility of the findings.