Figure 3.

DiY Formation in Aβ and vAβ via UV Photo-oxidation

(A–C) Freshly prepared Aβ and vAβ (50 μM) were incubated under UV. Fluorescence spectra were collected 5 min post-incubation using fluorescent excitation wavelength of 320 nm and emission collected between 340 and 600 nm, with DiY peak signal observed between 400 and 420 nm after 5 min of incubation (A), which increased following 2 h of incubation (B). Fluorescence intensity at 410 nm against time showed that incubation of the 2 h UV-exposed Aβ and vAβ samples in the dark resulted in further increase in DiY formation in the absence of the UV (C). The Aβ and vAβ samples that were not exposed to UV showed no DiY signal. A minimum of three independent experiments was repeated to ensure the reproducibility of the findings.