Figure 6.

Photo-oxidation of Pre-formed Aβ (aAβ) (24 h) Assemblies Slows Further Assembly of Aβ Oligomers/Protofibrils and Prolongs the Half-Life of the Oligomers

(A) DiY signal was detected in the oxidized aAβ+UV sample, which continued over time. Unoxidized aAβ samples showed no DiY signal.

(B) Th-T fluorescence showed that the unoxidized aAβ continues to assemble, whereas the oxidized aAβ+UV became significantly inhibited from further elongation up to 40 h.

(C) Dot blotting using NU-1 antibody reveals the presence of oligomers in the unoxidized and oxidized aAβ at the starting time point. However, the oligomers are still strongly detected in the oxidized aAβ+UV at 4 days, unlike in the unoxidized samples.

(D) TEM imaging at 4 days revealed the presence of fibrils in the unoxidized aAβ sample, whereas the oxidized aAβ+UV showed oligomers and a reduced number of fibrils. A minimum of three independent experiments was repeated to ensure the reproducibility of the findings. Scale bars, 500 nm