Figure 7.

Oxidized, DiY-Containing Aβ Assemblies Are Not Toxic to Cells

Differentiated SH-SY5Y neuroblastoma cells were incubated with UV oxidized or unoxidized vAβ or Aβ for 3 days, following which the percentage of dead cells was quantified using ReadyProbes Live/Dead Assay. The Aβ and vAβ samples were freshly prepared and UV oxidized for 2 h (Aβ+UV/vAβ+UV), or UV oxidized for 2 h followed by a further incubation on bench and in the dark for 24, 48, or 96 h, before being administered to cells. vAβ and Aβ samples not exposed to UV were used as reference. Only the unoxidized/assembling wild-type Aβ induced significant cell death. Experiments were repeated five times. ∗∗∗p < 0.001. p ≤  0.05 (∗), <0.01 (∗∗), <0.0001 (∗∗∗∗) and >0.05 was not significant. Error bars are expressed as ±SEM.