Figure 2.

Gal-3 impacts TPIN-SC proliferation, and sphere formation, tumorigenicity, and metastatic potential. TPIN-SCshScram or TPIN-SCshGal-3#5 were analyzed by IncuCyte for 88 h consecutively (A,B,D,F), and TPIN-SCkoGal-3 and TPIN-SCkoScram were analyzed 72 h consecutively (C,E,G), with image captured every 4 h. (A) Representative images of cell confluence and spheroids at day 3. Orange: mask to identify spheroids; objective: 10×; scale: 400 μm. (B,C) Cell proliferation was measured and reported as mean cell confluence percentage. (D,E) Spheroid proliferation was measured and reported as mean spheroid confluence percentage. (F,G) Spheroids dimension at day 3 was measured and reported as mean spheroid area (μm²). Statistical analyses were performed using the Student T-test. **p < 0.01; ****p < 0.0001.