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. 2020 Sep 2;319(4):L670–L674. doi: 10.1152/ajplung.00345.2020

Fig. 2.

Fig. 2.

Steady-state levels of mRNA transcripts encoding severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry receptors and co-receptors in airways and lung epithelial cells. A: steady-state mRNA levels were determined by real-time RT-PCR for Ace2, Tmprss1, Tmprss2, and Tmprss11d in cDNA prepared from adult C57BL/6J mouse alveolar epithelial type II cells (n = 4 independent cell-cultures per group) incubated on an air-liquid interface under room air (21% O2: yellow bars) or hyperoxia (85% O2: blue bars) conditions for 24, 48, or 72 h. B: steady-state mRNA levels were similarly determined for ACE2, TMPRSS1, TMPRSS2, and TMPRSS11D in adult human nasal, tracheal, esophageal, bronchial, and alveolar epithelial cells (n = 4 independent cell-cultures per group) incubated on an air/liquid interface under room air (21% O2: yellow bars) or hyperoxia (85% O2: blue bars) conditions for 48 h. Data reflect mean ΔCt ± SD. Pairwise comparisons were made between the 21% O2 and 85% O2 groups by unpaired Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. n.d., not detected. ACE2, angiotensin I converting enzyme 2; POLR2A/Polr2a, RNA polymerase II subunit A; TMPRSS, transmembrane serine protease.