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Effect of phloretin on LKB1/AMPK/Nrf2/HO-1 activity in C2C12 cells. Cells at a density of 70% were treated (or not treated) with 10 μmol/L phloretin and 500 μmol/L H2O2. (A) Protein expression of HO-1 and phosphorylation levels of (B) p-Nrf2, (C) p-AMPK, and (D) p-LKB1 were detected. Cells were cultured with compound C (10 μmol/L), an inhibitor of AMPK, for 3 h in (B).
