FIGURE 3.

(A) LTA binding affinity of peptides and the antibiotic was determined using the BODIPY-TR cadaverine (BC) fluorescent dye displacement assay. The changes in fluorescence intensity were monitored at an excitation wavelength of 580 nm and an emission wavelength of 620 nm. The experiment was repeated three times independently. (B) Cytoplasmic membrane depolarization of S. aureus was monitored using membrane potential dye diSC₃-5. (C) The fluorescence intensity of propidium iodide incubated with S. aureus 29213 treated by 1/2, 1, and 2 × MIC peptides were measured. Exponential-phase S. aureus cells were treated with melittin (blue), and S2 (red).