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. 2020 Sep 11;8:574619. doi: 10.3389/fcell.2020.574619

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Copyright © 2020 Iwashita, Nomura, Suetsugu, Matsuzaki, Kojima and Kosodo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of tissue stiffness in juvenile songbird brains in different fixatives. (A) Representative images of juvenile songbird brain slices: (a) acute, (b) glyoxal-fixed, and (c) PFA-fixed. (B) Comparison of stiffness in P (a) and Str (b) in different fixatives. (a′ and b′) Magnified views of insets in (a and b), respectively. (C) Comparison of stiffness between P and Str in each fixative. P, pallium; Str, striatum; scale bar: 1 mm. One-way ANOVA with Tukey post hoc test; P < 0.0001 (****) for (B). Two-tailed unpaired t-test; P < 0.05 (*), P < 0.001 (***), and P < 0.0001 (****) for(C). Error bars in graphs are represented as the mean ± SEM.