FIGURE 4.

Citrullinated H3 in supernatants of stored canine packed red blood cell units. Representative Western blot analysis of citrullinated histone H3 in 1 pair of canine nonleukoreduced (NLR) and leukoreduced (LR) packed red blood cell units from the same dog over the 42 day storage period. The 17.4 kDa protein ladder band is shown. The labeled positive control is citrullinated histone H3 purified from canine septic abdominal fluid. The entire width of the blot is included, but the area of the gel above the 21 kDa molecular marker has been cropped out of the image for clarity