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. 2020 Sep 3;34(5):1894–1902. doi: 10.1111/jvim.15876

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© 2020 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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DNA release from healthy canine neutrophils evaluated by fluorescence. Neutrophils isolated from healthy dog blood were incubated with and without supernatants from nonleukoreduced (NLR, A) and leukoreduced (LR, B) stored packed red blood cell units and Sytox green added to detect extracellular DNA. Data is presented as fold change in fluorescence compared to unstimulated neutrophils (mean ± SD). Supernatants from 5 NLR and LR units were assessed for ability to induce DNA release from isolated canine neutrophils. Assays were performed in triplicate