Figure 4.

Knockdown of CCND3 inhibits the colony forming ability and viability of glioblastoma cells. (A) Dual luciferase reporter gene assay was performed to determine whether miR-138-5p directly targeted CCND3 in cells co-transfected with p-CCND3-wt or p-CCND3-mut and either miR-138-5p mimics. The mean of the results from cells transfected with miR-138-5p NC and p-con was set as 100. **P<0.01. The silencing effect of CCND3 knockdown using siRNA was analyzed using (B) reverse transcription-quantitative PCR and (C) western blotting in U87 and U251 cell lines transfected with si-CCND3 or si-NC. (D) Colony forming ability of U87 and U251 cells transfected with si-CCND3 or si-NC was analyzed. (E) Cell viability of U87 and U251 cells transfected with si-CCND3 or si-NC was analyzed using a Cell Counting Kit-8 assay. *P<0.05, **P<0.01 vs. si-NC. A one-way ANOVA followed by a Tukey's test post hoc was used in parts (A and E) while an unpaired Student's t-test was used in parts (B-D) CCND3, cyclin D3; miR, microRNA; wt, wild-type; mut, mutant; p, plasmid; con, control; si/siRNA, small interfering RNA; NC, negative control; OD, optical density.